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UK Health Security Agency

HRA-16

HRA-16

Catalogue No.

06061905

Cell Line Name

HRA-16

Cell Line Description

Established from the rectum of a 56 year-old male patient. Epithelial-like moderately differentiated adenocarcinoma. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Rectum

Karyotype

Not specified

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Cells grow as small dense islands and will not form a monolayer. Recommended that the cell line should be subcultured approximately every 7-14 days.

Seed cells at 2-4x10,000 cells/cm² and use 0.05% trypsin or trypsin/EDTA as a dissociation reagent. Detached cells will often remain in clumps which may be further disaggregated by repeated pipetting of the cells.

Recommended growth conditions 7.5% CO2; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road, London, W12 0NN

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

S. C. Kirkland and I. G. Bailey. (1986) Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer, 53, 6, 779-85.

Bibliography

Not Available

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HRA-16 (ECACC 06061905).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.