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HS-Sultan

HS-Sultan

Catalogue No.

87012701

Cell Line Name

HS-Sultan

Cell Line Description

It has been clearly established that HS-Sultan is a subclone of the Burkitt lymphoma derived cell line JIYOYE and not a myeloma cell line. (see Drexler HG et al., 2001 Cross-contamination: HS-Sultan is not a myeloma but a Burkitt lymphoma cell line. Blood 98(12):3495-6.PMID: 11732505. Drexler HG et al., 2003 False leukemia-lymphoma cell lines: an update on over 500 cell lines. Leukemia. 17(2):416-26. PMID: 12592342)

Characteristics

Products

Immunoglobulin G (IgG) kappa chains in spinner culture. Endoplasmic reticulum in stationary culture.

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,11
D5S818: 12
D7S820: 8,10
D13S317: 12
D16S539: 10,11
TH01: 7,9
TPOX: 6,8
vWA: 15,19

Karyotype

Hyperdiploid modal no=48

Applications

Tumourigenicity studies

Disease

Burkitt Lymphoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. A density of 3x1,000,000 cells/ml may be achieved.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Nature 1974;250:507; J Immunol 1976;118:1480

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HS-Sultan (ECACC 87012701).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.