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UK Health Security Agency

IEC 6

IEC 6

Catalogue No.

88071401

Cell Line Name

IEC 6

Cell Line Description

IEC 6 (Intenstinal Epithelial Cell 6) was developed by Quaroni et al in 1979 from the successful culture of small fragments of rat small intestine. The cell line consists of a homogenous population of epithelioid cells that recapitulate the characteristics of normal cells cultured in vitro. They are thought to represent undifferentiated small intestinal crypt cells. These features are prolonged by culturing the cells in reduced (5%) FBS and 0.1 IU/ml Insulin. The original publication (J Cell Biol 1979;80:248) suggests that the cell line has finite lifespan of 40-50 passages. Typically vials of ECACC IEC 6 are supplied at passage numbers of 20-30. The cells should therefore be able to be passaged at least another 10 times (approximately 20 population doublings) by end users. This is a conservative estimate and cultures may last significantly longer. We advise end users to monitor their cultures and ensure they  retain the homogenous epithelioid characteristics reported in the original publication and an approximate 24 hour doubling time.

Characteristics

Tissue of Origin

Intestine

Karyotype

Not specified

Applications

Small bowel biology

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Cell Biol 1979;80:248

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: IEC 6 (ECACC 88071401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.