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IIC9 (CH0)

IIC9 (CH0)

Catalogue No.

00030814

Cell Line Name

IIC9 (CH0)

Cell Line Description

This cell line is a selected subclone (IIC9) of the CHEF 18 Chinese Hamster embryo cell line

Characteristics

Receptors

PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF

Tissue of Origin

Embryo

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.05% trypsin /EDTA; 37°C; 5% CO₂.

Culture Medium

MEM/F12 1:1 + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Growth Mode

Adherent

Additional Info

Depositor

Dr D M Raben, Department of Medicine, John Hopkins University School of Medicine, Baltimoe, Maryland. 21205

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Journal of Biological Chemistry 1993 268 9215-9222. PubMed=7088192; DOI=10.1038/298476a0; Low D.A., Scott R.W., Baker J.B., Cunningham D.D.; "Cells regulate their mitogenic response to thrombin through release of protease nexin."; Nature 298:476-478(1982).

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: IIC9 (CH0) (ECACC 00030814).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.