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IM 9

IM 9

Catalogue No.

86051302

Cell Line Name

IM 9

Cell Line Description

Derived from bone marrow removed from a female patient with multiple myeloma. The cells synthesise IgG at a rate of 2.4ug/1,000,000 cells/day and have receptors for insulin, calcitonin and human growth hormone. They also exhibit surface markers and receptor sites characteristic of B lymphocytes. EBV Transformed

Characteristics

Products

Immunoglobulin G (IgG)

Tissue of Origin

Bone marrow

Karyotype

2n = 46

Applications

Biosynthetic and surface marker studies

Disease

None Stated

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 2-4x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1,000,000 cells/ml.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Ann NY Acad Sci 1972;190:221; Nature 1974;251:443

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: IM 9 (ECACC 86051302).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.