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KC

KC

Catalogue No.

90070550

Cell Line Name

KC

Cell Line Description

Resistant to osmolarity changes within 225-400 mosmols and to chloride levels 4-80mM; the usual Na/K ratio of 1.6 can be both increased and decreased considerably. Viruses present are non-pathogenic but can be induced when inoculated into a new host.

Characteristics

Tissue of Origin

Embryo

Karyotype

Not specified

Applications

Virus studies: rhabdovirus Drosophila X, picornavirus Drosophila C

Disease

None Stated

Culture Conditions

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml. Cells may adhere to the tissue culture flask, shake gently to dislodge; 25°C; No CO₂.

Culture Medium

Schneider's Drosophila medium + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Semi-adherent

Additional Info

Depositor

Dr J Sinclair, Dept. of Medicine, Addenbrookes Hospital, Cambridge

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Adv Cell Culture 1981;1:125; Somatic Cell and Mol Gen 1985;11:63; Cell 1989;57:1017; Genetics and Develop 1989;3:882

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: KC (ECACC 90070550).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.