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KF1

KF1

Catalogue No.

10072801

Cell Line Name

KF1

Cell Line Description

The KF1 cell line was developed from Koi carp (Cyprinus carpio koi) fin.

Characteristics

Tissue of Origin

Fin

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 25°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). 10mM HEPES can be included if a CO₂ incubator is not available.

Growth Mode

Adherent

Additional Info

Depositor

Dr Chris Hartley, Henderson Morley plc, Metropolitan House, 2 Salisbury Road, Moseley, Birmingham B13 8JS (Originator: Dr Ronald Hedrick, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA)

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

R. P. Hedrick, O. Gilad, S. Yun, J. V. Spangenberg, G. D. Marty, R. W. Nordhausen, M. J. Kebus, H. Bercovier, and A. Eldar (2000) A Herpesvirus Associated with Mass Mortality of Juvenile and Adult Koi, a Strain of Common Carp. Journal of Animal Health 12: 44-57.

Bibliography

D. Pokorova, T. Vesely, V. Piackova, S. Reschova, J. Hulova (2005) Current knowledge on koi herpesvirus (KHV): a review Vet. Med. Czech, 50, (4): 139-147

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: KF1 (ECACC 10072801).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.