Catalogue No.
19072503
KMX-1
KMX-1
Cell Line Name
KMX-1
Cell Line Description
KMX-1 was developed by immunising mice with purified Physarum polycephalum, a slime mould. P. polycephalum is an ideal model organism to investigate cell biological or biophysical details. In this case it has been used to investigate the variability in tubulin.
BALB/c mice were inoculated with 20 - 50pg of purified P. polycephalum per injection. The mouse spleen cells were then fused with the myeloma Sp2-0/Ag14
General Info
Fusion Species
BALB/c mice were inoculated with 20 - 50pg of purified P. polycephalum per injection. The mouse spleen cells were then fused with the myeloma Sp2-0/Ag14.
Target (Reactivity)
Anti β-tubulin
Also known as
KMX1
Unique to ECACC
Yes
Nagoya Status
Not Applicable
Release Conditions
Characteristics
Myeloma
Sp2-0/Ag14
Immunogen
Physarum polycephalum amoebal tubulin
Antibody Isotype
IgG2b
Morphology
Spherical
Tissue of Origin
Hybrid
Culture Conditions
Cell Type
Hybridoma
Subculture Routine
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C On resuscitation, a centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes.Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Place culture flask flat and observe regularly until viable proliferating cells are seen.
When recovering hybridoma cultures from frozen it is not unusual for growth to be slower than expected initially and there may be an observed decrease in viability. Establishment of an actively proliferating culture may take up to 2 weeks.
Culture Medium
DMEM + 2mM glutamine + 10% FBS
Often hybridoma cultures may benefit from being re-suspended with media supplemented with 20% FBS in the early critical stage of culture establishment immediately post resuscitation. Once the culture is established the FBS can be reduced to 10%.
Growth Mode
Suspension
Additional Info
Depositor
Keith Gull, University of Oxford
Country of Origin
United Kingdom
Applications
Extremely widely reacting anti-β tubulin antibody across most species. Studies of individual isotype expression of a multi-tubulin family.
Assays
Immunohistochemistry
Western Blot
GMO Status
Not Applicable
Hazard Group (ACDP)
2
References
References
Birkett, C.R., Foster, K.E., Jonson, L. and Gull, K. (1985): Use of monoclonal antibodies to analyse the expression of a multi-tubulin family. FEBS Letters, 187 (2):211-218.
Bibliography
Roobol, A., Wilcox, M., Paul, E.C.A. and Gull, K. (1983) Eur. J. Cell Biol. 33, 24-28.
Burland, T.G., Gull, K., Schedl, T., Boston, R.S. and Dove, W.F. (1983) J. Cell Biol. 97, 1852-1859.
Schedl, T., Burland, T.G., Gull, K. and Dove, W.F. (1984) J. Cell Biol. 99, 155-165.
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: KMX-1 (ECACC 19072503)
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.