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KT15

KT15

Catalogue No.

19031501

Cell Line Name

KT15

Cell Line Description

test

General Info

Fusion Species

Rat x Mouse Hybridoma

Target (Reactivity)

CD8 α

Also known as

KT-15

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Myeloma

NS-0

Immunogen

T cell clone, C6, H-2KK restricted antigen specific CTL

Immunological Donor

Rat spleen

Antibody Isotype

Rat IgG2aλ

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

When resuscitating from frozen, cells should be seeded at 0.5 x 10⁶ cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 10⁵ cells/ml. Maintain cultures between 3-9 x 10⁵ cells/ml for maximum growth; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM L-glutamine + 10% Foetal Bovine Serum (FBS) + 10 % Hepes

Growth Mode

Suspension

Additional Info

Depositor

LifeArc on behalf of MRC (part of UKRI).MRC are the owners of the material. LifeArc manage the commercialisation of the IP.

Country of Origin

United Kingdom

Applications

Flow Cytometry

Assays

This monoclonal antibody KT15 reacts with a non-polymorphic epitope on the mouse CD8 α chain (mouse Ly2.1 and Ly2.2 cells).

Hazard Group (ACDP)

2

References

References

Tomonari K, Spencer S. Epitope-specific binding of CD8 regulates activation of T cells and induction of cytotoxicity. Int Immunol. 1990;2(12):1189-94. PubMedPMID: 1708676.Tomonari K, Lovering E, Fairchild S, Spencer S. Two monoclonal antibodies specific for the T cell receptor V alpha 8. Eur J Immunol. 1989 Jun;19(6):1131-5. PubMed PMID: 2526741.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: KT15 (ECACC 19031501).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.