KT15
KT15
Cell Line Name
Cell Line Description
test
General Info
Fusion Species
Target (Reactivity)
Also known as
Release Conditions
Characteristics
Myeloma
NS-0
Immunogen
T cell clone, C6, H-2KK restricted antigen specific CTL
Immunological Donor
Antibody Isotype
Morphology
Tissue of Origin
Culture Conditions
Cell Type
Subculture Routine
When resuscitating from frozen, cells should be seeded at 0.5 x 10⁶ cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 10⁵ cells/ml. Maintain cultures between 3-9 x 10⁵ cells/ml for maximum growth; 5% CO₂; 37°C.
Culture Medium
RPMI 1640 + 2mM L-glutamine + 10% Foetal Bovine Serum (FBS) + 10 % Hepes
Growth Mode
Additional Info
Depositor
Country of Origin
Applications
Assays
This monoclonal antibody KT15 reacts with a non-polymorphic epitope on the mouse CD8 α chain (mouse Ly2.1 and Ly2.2 cells).
Hazard Group (ACDP)
References
References
Tomonari K, Spencer S. Epitope-specific binding of CD8 regulates activation of T cells and induction of cytotoxicity. Int Immunol. 1990;2(12):1189-94. PubMedPMID: 1708676.Tomonari K, Lovering E, Fairchild S, Spencer S. Two monoclonal antibodies specific for the T cell receptor V alpha 8. Eur J Immunol. 1989 Jun;19(6):1131-5. PubMed PMID: 2526741.
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: KT15 (ECACC 19031501).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.