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KYSE-270

KYSE-270

Catalogue No.

94072021

Cell Line Name

KYSE-270

Cell Line Description

Established from the well differentiated invasive oesophageal squamous cell carcinoma resected from the middle intra-thoracic oesophagus of a 79-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC.

Characteristics

Tissue of Origin

Oesophagus

Karyotype

Hypotriploid with 16% polyploidy

Disease

Squamous Cell Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split confluent cultures 1:5 every second day using trypsin/EDTA; 5% CO₂; 37°C. Doubling time: 24 hours. Recommended seeding density: 2.5 x 10,000 cells/cm².

Culture Medium

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr Y Shimada, Kyoto University School of Medicine, Kyoto, Japan

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Shimada Y, Imamura M, Wagata T, Yamaguchi N, Tobe T.1992 Characterization of 21 newly established esophageal cancer cell lines. Cancer. 69(2):277-84. Erratum in: Cancer 1992 70(1):206 PMID: 1728357.

Bibliography

Not Available

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: KYSE-270 (ECACC 94072021).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.