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UK Health Security Agency

L5178Y tk+/- 3.7.2c (IVGT)

L5178Y tk+/- 3.7.2c (IVGT)

Catalogue No.

12080201

Cell Line Name

L5178Y tk+/- 3.7.2c (IVGT)

Cell Line Description

The L5178Y tk+/- 3.7.2c (IVGT) mouse lymphoma cell line was derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene treatment. The cell line is tumourigenic in DBA/2 mice. The cell line can be used in genotoxicity tests including gene mutation assays at the tk and other loci and micronucleus induction.

Characteristics

Tissue of Origin

Thymus

Morphology

Small rounded cells

Karyotype

Modal chromosome number of 40 and a composite karyotype 40 X0 der 5t(5;15), der 9t(9;6) Robertsonian fusion 12 and 13, þ15(t15;5), þ15(t15;18), 15q, der18t(18;6) (same as Sawyer JR et al. Environ Mol Mutagen 2006; 47: 127-131). 2 copies of chromosome 11.

Disease

Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures in logarithmic growth phase between 3 x 10⁴ and 1 x 10⁶ cells/ml.

Culture Medium

Fischer's medium + 2mM Glutamine + 1% Sodium pyruvate + 10% heat-inactivated Horse Serum (HS)

Growth Mode

Suspension

Additional Info

Depositor

Dr M O'Donovan, AstraZeneca R & D, Innovative Medicines, GSA, Mereside, Alderley Park, Macclesfield SK10 4TG. UK. Originator: D Clive, Burroughs Wellcome Co. Research, Triangle Park, North Carolina, USA.

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Sawyer JR, Binz RL, Wang J, Moore MM. 2006 Multicolor spectral karyotyping of the L5178Y Tk+/- -3.7.2C mouse lymphoma cell line. Environ Mol Mutagen.47(2):127-31.PMID: 16247762

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: L5178Y tk+/- 3.7.2c (IVGT) (ECACC 12080201).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.