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UK Health Security Agency

LA-N-2

LA-N-2

Catalogue No.

06041202

Cell Line Name

LA-N-2

Cell Line Description

Established by Seeger et al., (1977) from the primary site of a noncatecholamine-producing neuroblastoma of a 3-year-old female with clinical Stage IV neuroblastoma. The culture consists mostly of large, elongated cells with processes with some small round cells adhering to the elongated cells. The cells are tumourigenic in nude mice.

Characteristics

Tissue of Origin

Neuroblastoma

Morphology

Large elongated cells + small round cells in clusters

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10,12
D5S818: 11,12
D7S820: 8,10
D13S317: 11,12
D16S539: 9,10
TH01: 9.3
TPOX: 8
vWA: 16,18

Karyotype

Modal no. 73, range 46 - 73

Disease

Neuroblastoma

Culture Conditions

Cell Type

Neuronal

Subculture Routine

For routine maintenance, split cultures after they have become dense i.e. once every 3-4 weeks at a 1:20 to 1:50 ratio; 8% CO₂; 37°C. The culture consists of large elongated cells with processes and small round cells. The latter tend to grow in dense clusters on the former and detach readily. Most cells are moderately adherent. Remove attached cells from the substrate with trypsin/EDTA. Cells will detach in 5-10 minutes. When resuscitated from a frozen ampoule cells may appear dead after a day but reattach and resume growth within 2-3 days. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes.

Culture Medium

EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr Robert A Ross, Laboratory of Neurobiology, Department of Biological Sciences, Fordham University, New York, USA

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Seeger RC, Rayner SA, Banerjee A, Chung H, Laug WE, Neustein HB, Benedict WF 1997 Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37(5):1364-71 PMID: 856461

Bibliography

Ross RA, Biedler JL, Spengler BA, Reis DJ 1981 Neurotransmitter-synthesizing enzymes in 14 human neuroblastoma cell lines. Cell Mol Neurobiol. 1(3):301-11 PMID: 6125265

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LA-N-2 (ECACC 06041202).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.