Catalogue No.
06041203
LA1-55n
LA1-55n
Cell Line Name
LA1-55n
Cell Line Description
Derived from LA1-5s a clonal subline of LA-N-1. LA-N-1 was derived from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma. This cell line shows little or no transdifferentiation to the S phenotype.
LA-N-1 (ECACC Catalogue number 06041201), LA1-55n (ECACC Catalogue number 06041203) and LA1-5s (ECACC Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.
General Info
Species
Human
Links
Cellosaurus: CVCL_2548
Release Conditions
Characteristics
Tissue of Origin
Neural
Morphology
Fusiform
Disease
Neuroblastoma
Culture Conditions
Cell Type
Fibroblast-like
Subculture Routine
On resuscitation, it is recommended that the cells should be seeded between 2-4 x 104 cells/cm2. The cultures should be left to become dense (approx. 6-8 days) before subculture is performed. Cells are weakly to moderately adherent and can be removed by washing the monolayer with PBS. In flasks, repeated pipetting of the PBS over the monolayer should detach the cells. In cell stacks, it is suggested that 2 layers are not exceeded as repeated rocking of the PBS over the monolayer is required and may take over 10 mins to detach. Alternatively, 0.1% trypsin can be used to detach the cells, however, some will be lost during the PBS wash.
When seeded into new flasks cells reattach readily. The population doubling time is approximately 2 days; saturation density is >1,000,000 cells/cm2. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH.
Recommended growth conditions: ~8% CO2; 37°C.
Culture Medium
EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Growth Mode
Semi-adherent aggregates
Additional Info
Depositor
Dr Robert A Ross, Laboratory of Neurobiology, Department of Biological Sciences, Fordham University, New York, USA
Country of Origin
United States
Hazard Group (ACDP)
Hazard Group (ACDP) 2
Applications
References
Ciccarone V, Spengler BA, Meyers MB, Biedler JL, Ross RA 1989 Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.Cancer Res.;49(1):219-25 PMID: 2535691
Bibliography
Spengler BA, Lazarova DL, Ross RA, Biedler JL. 1997 Cell lineage and differentiation state are primary determinants of MYCN gene expression and malignant potential in human neuroblastoma cells. Oncol Res; 9(9):467-76 PMID: 9495452
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: LA1-55n (ECACC 06041203).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.