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UK Health Security Agency

LA1-5s

LA1-5s

Catalogue No.

06041204

Cell Line Name

LA1-5s

Cell Line Description

LA1-5s is a clonal subline of the cell line LA-N-1 which was established by Seeger et al., (1977) from the primary site of a noncatecholamine-producing neuroblastoma of a 3-year-old female with clinical Stage IV neuroblastoma. The LA1-5s culture consists of large, flat, and tightly adherent cells. They are precursors of Schwann cell/glia/melanocyte lineages arising from the neural crest. This cell line is not tumourigenic in nude mice at a 1,000,000 cell inoculum. The cells can transdifferentiate to a neuroblastic phenotype. The N-myc gene has been reported to be amplified but exhibits very low expression.LA-N-1 (ECACC Catalogue number 06041201), LA1-55n (ECACC Catalogue number 06041203) and LA1-5s (ECACC Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Characteristics

Tissue of Origin

Neural

Karyotype

Not specified

Disease

Neuroblastoma

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

For routine maintenance, split cultures every 2-3 weeks at a 1:10 to 1:100 ratio; 8% CO₂; 37°C. The cells are large, flat, fibroblast-like cells that adhere strongly to the substrate. They form a swirling monolayer with no mounding or multilayering when in culture for only a short time, but will increase in density in the stationary phase as the culture is maintained. The cells can be removed from the substrate by rinsing with PBS, then incubating with EDTA/trypsin (at room temperature). Cells will detach in 5-10 minutes. Cells will reattach within several hours after seeding into a new flask. Population doubling time is approximately 2 days. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes, but may also give rise to neuroblastic variants.

Culture Medium

EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr Robert A Ross, Laboratory of Neurobiology, Department of Biological Sciences, Fordham University, New York, USA

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Ciccarone V, Spengler BA, Meyers MB, Biedler JL, Ross RA 1989 Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.Cancer Res.;49(1):219-25 PMID: 2535691

Bibliography

Spengler BA, Lazarova DL, Ross RA, Biedler JL. 1997 Cell lineage and differentiation state are primary determinants of MYCN gene expression and malignant potential in human neuroblastoma cells. Oncol Res; 9(9):467-76 PMID: 9495452

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LA1-5s (ECACC 06041204).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.