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UK Health Security Agency

LB182

LB182

Catalogue No.

98062317

Cell Line Name

LB182

Cell Line Description

Derived from mononuclear cells from peripheral blood of a 51 year old normal male. Cells were subsequently transformed with EBV. Fibroblasts designated 149BR (ECACC catalogue no. 90011807) have been isolated from the same individual . This has been confirmed by STR profiling at ECACC. T cells from the same sample have been used for cellular radiosensitivity studies and cell survival analysis after UV-irradiation.

Characteristics

Tissue of Origin

Blood

Karyotype

Not specified

Applications

Mutation studies, Toxicity analysis

Disease

None Stated

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Green MH, Arlett CF, Cole J, Harcourt SA, Priestley A, Waugh AP, Stephens G, Beare DM, Brown NA, Shun-Shin GA 1991 Comparative human cellular radiosensitivity: III. Gamma-radiation survival of cultured skin fibroblasts and resting T-lymphocytes from the peripheral blood of the same individual. Int J Radiat Biol. 59(3):749-65 PMID: 1672363.

Bibliography

Arlett CF, Harcourt SA, Cole J, Green MH, Anstey AV. 1992 A comparison of the response of unstimulated and stimulated T-lymphocytes and fibroblasts from normal, xeroderma pigmentosum and trichothiodystrophy donors to the lethal action of UV-C. Mutat Res. 273(2):127-35 PMID: 1372096.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LB182 (ECACC 98062317).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.