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UK Health Security Agency

LB199

LB199

Catalogue No.

98062321

Cell Line Name

LB199

Cell Line Description

LB199 was derived from mononuclear cells from a peripheral blood sample from a 16 year old male. The patient was diagnosed with trichothiodystrophy and was shown to have a severe deficiency in excision repair of UV-irradiation damage. The defect was able to complement all known Xeroderma pigmentosum complementation groups and was not intragenic. This cell line is therefore a representative of a new excision-repair complementation group. The cell line was established by subsequent EBV transformation. Fibroblasts from the same individual have been designated TTD1BR. EBV-transformed lymphoblastoid cells from the mother are available as LB198 (ECACC catalogue no. 98062320).

Characteristics

Tissue of Origin

Blood

Karyotype

Not specified

Applications

DNA repair deficiency studies

Disease

None Stated

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; dilute every 1 -2 days to 3 x 100,000 cells/ml; 5% CO₂; 37°C. Cells form floating aggregates.

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Broughton BC, Lehmann AR, Harcourt SA, Arlett CF, Sarasin A, Kleijer WJ, Beemer FA, Nairn R, Mitchell DL.1990 Relationship between pyrimidine dimers, 6-4 photoproducts, repair synthesis and cell survival: studies using cells from patients with trichothiodystrophy. Mutat Res. 235(1):33-40 PMID: 2300071.

Bibliography

Arlett CF, Harcourt SA, Cole J, Green MH, Anstey AV. 1992 A comparison of the response of unstimulated and stimulated T-lymphocytes and fibroblasts from normal, xeroderma pigmentosum and trichothiodystrophy donors to the lethal action of UV-C. Mutat Res. 273(2):127-35 PMID: 1372096. Stefanini M, Vermeulen W, Weeda G, Giliani S, Nardo T, Mezzina M, Sarasin A, Harper JI, Arlett CF, Hoeijmakers JH, et al. 1993 A new nucleotide-excision-repair gene associated with the disorder trichothiodystrophy. Am J Hum Genet. 53(4):817-21 PMID: 8213812.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LB199 (ECACC 98062321).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.