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UK Health Security Agency

LIM1863

LIM1863

Catalogue No.

17020201

Cell Line Name

LIM1863

Cell Line Description

LIM1863 is a cell line derived from a sporadic colorectal cancer (CRC), from a 74- year-old female patient. The cells grow as organoids and are organized around a central lumen. Both morphologically mature columnar and goblet cells are present in the organoids. LIM1863 will adhere in response to TGF-beta

Characteristics

Tissue of Origin

Ileoceacal valve

Morphology

LIM1863 grows as floating organoids

Disease

Adenocarcinoma

Culture Conditions

Subculture Routine

Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Medium Renewal: 3 times per week. Subcultivation ratio: 1:4, Split once a week at this optimal ratio. To subculture, disperse the organoids into smaller clusters by vigorous pipetting and then split 1:4 to seed. Note: this cell line does not tolerate low density. If there are too few organoids in the flask to grow well they may need to be concentrated into a smaller volume. To obtain an estimated cell count, remove an aliquot of the cells suspension and after PBS wash, use 0.05% Trypsin/EDTA at 37°C for 5 minutes with mechanical dispersing before cell count. (Seeding density 2-4 x10⁴ cells/cm²). Culture conditions: Incubate the culture at 37°C with 5% CO₂. Cryoprotectant Medium: 10% DMSO + 90% FCS

Culture Medium

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES)+10% FBS+ 1ug/ml Hydrocortisone + 0.6ug/ml Insulin + 10uM Thioglycerol

Growth Mode

Suspension

Additional Info

Depositor

Professor Tony Burgess - Ludwig Institute for Cancer Research, Australia

Country of Origin

Australia

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Whitehead RH, Jones JK, Gabriel A, Lukies RE. A new colon carcinoma cell line (LIM1863) that grows as organoids with spontaneous differentiation into crypt-like structures in vitro. Cancer Res 1987; 47:2683-9. PMID: 3567898

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LIM1863 (ECACC 17020201).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.