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UK Health Security Agency

LIM2537

LIM2537

Catalogue No.

21102106

Cell Line Name

LIM2537

Cell Line Description

LIM2537 is an adherent cell line derived from a primary colonic carcinoma from a 73-year-old male patient.

 

This cell line is distributed by ECACC on behalf of CellBank Australia, Children's Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by Cell Bank Australia. Cell Bank Australia reference number: CBA-0168

Characteristics

Tissue of Origin

Colon

DNA profile (STR Profile)

A33 negative, MSI


Truncated APC (heteozygous stop at aa 1472), mutated p53 (aa G245D), mutated B-Raf (aa V600E), mutated PI3K (23 aa deletion in p85α).

Applications

 

 

 

Disease

Carcinoma

Culture Conditions

Cell Type

Merkel cell carcinoma

Subculture Routine

Remove protective cryoflex layer around the ampoule prior to thawing.


Thaw the ampoule by gently agitating in a 37oC waterbath; thawing should be rapid (around 2 minutes).


A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Culture Medium

Medium Renewal: 3 times per week.


Subcultivation ratio: 1:8, Seeding density 1-2 x104 cells/cm2


Split subconfluent cultures (70%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.


Culture conditions: Incubate the culture at 37oC with 5% CO2.


Cryoprotectant Medium: 10% DMSO + 90% FCS

Growth Mode

Adherent

Additional Info

Depositor

Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne - Australia

Country of Origin

Australia

GMO Status

Not Applicable

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

References Philp AJ, Campbell IG, Leet C, Vincan E, Rockman SP, Whitehead RH, Thomas RJ, Phillips WA.
The Phosphatidylinositol 3′-kinase p85α Gene Is an Oncogene in Human Ovarian and Colon Tumors Cancer Res. 2001 Oct 15;61(20):7426-9. PubMed 11606375
Zhang H.H., Walker F., Kiflemariam S., Whitehead R.H., Williams D., Phillips W.A., Mikeska T., Dobrovic A., Burgess A.W. Selective inhibition of proliferation in colorectal carcinoma cell lines expressing mutant APC or activated B-Raf.
Int.J.Cancer 125(2):297-307 PubMed 19378335

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LIM2537 (ECACC 21102106).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.