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UK Health Security Agency

LIM2550

LIM2550

Catalogue No.

12070504

Cell Line Name

LIM2550

Cell Line Description

LIM2550 is derived from an adenocarcinoma of the colon. This cell line is distributed by ECACC on behalf of CellBank Australia, Children's Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by Cell Bank Australia. Cell Bank Australia reference number: CBA-0169.

Characteristics

Tissue of Origin

Colon

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial - cells grow as floating and loose aggregates with some adherent cells.

Subculture Routine

Optimal split ratio 1:4-1:8 (seeding density 1-2 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM2550 requires the growth medium to be changed 3 times each week. Feed and passage the cells as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).

Culture Medium

RPMI1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6µg/ml, Hydrocortisone 1µg/ml, 1-Thioglycerol 10 µM

Growth Mode

Adherent

Additional Info

Depositor

Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne, Australia

Country of Origin

Australia

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Zhang HH, Walker F, Kiflemariam S, Whitehead RH, Williams D, Phillips WA, Mikeska T, Dobrovic A, Burgess AW. 2009 Selective inhibition of proliferation in colorectal carcinoma cell lines expressing mutant APC or activated B-Raf. Int J Cancer.125 (2):297-307 PMID: 19378335.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LIM2550 (ECACC 12070504).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.