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UK Health Security Agency

m17.ASC

m17.ASC

Catalogue No.

11112201

Cell Line Name

m17.ASC

Cell Line Description

m17.ASC is a spontaneously immortalised multipotent mesenchymal cell line capable of acquiring adipocyte, chondrocyte, osteocyte and cardiomyocyte phenotypes when cultured with appropriate differentiation factors. m17.ASC provides a valuable tool for in vitro biomimetic studies; assessing growth technologies such as 3-D structures, biochemical cues (selected growth factors and hormones) and physical forces (electrical, mechanical and topographical). It can be used to screen compounds that induce or inhibit proliferation and/or differentiation in tissue specific phenotypes.

Characteristics

Receptors

Sca-1, c-kit/CD117, nestin, nucleostemin, CD44 and CD106

Tissue of Origin

Adipose

Karyotype

Normal diploid

Disease

None Stated

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (90%) approximately 1:6 with media changes or subculture every 2-3 days seeding at 0.5-4 x 10⁴ cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Claycomb medium (Sigma 51800C) + 2mM glutamine + 10% FCS

Growth Mode

Adherent

Additional Info

Depositor

Professor M Prat, Head of the Laboratory of Histology, Department of Medical Sciences, Universita del Piemonte Orientale, Italy

Country of Origin

Italy

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Zamperone A, Pietronave S, Merlin S, Colangelo D, Ranaldo G, Medico E, Di Scipio F, Berta GN, Follenzi A, Prat M. 2013 Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue. Stem Cells Dev. 22(21):2873-84. PMID: 23777308

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: m17.ASC (ECACC 11112201).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.