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UK Health Security Agency

MDCK-Protein Free

MDCK-Protein Free

Catalogue No.

02050101

Cell Line Name

MDCK-Protein Free

Cell Line Description

MDCK cell line (ECACC catalogue no. 84121903) adapted to grow in the protein-free medium 'MDCK Protein-free Medium' (Sigma 14581C). Stirred culture (or Ultra low attachment flasks Corning 3814) is recommended, cells grow in suspended clumps.

Discontinued Message

Cell line temporarily discontinued pending investigation.

Characteristics

Tissue of Origin

Kidney

Karyotype

Not specified

Applications

Propagation of viruses in the absence of animal-derived proteins

Disease

None Stated

Culture Conditions

Subculture Routine

Stirred culture (or Ultra low attachment flasks Corning 3814) is recommended, cells grow in suspended clumps.Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C.

Culture Medium

MDCK Protein-free medium (Sigma 14581C) + 3.3mM Glutamine. When freezing cells down use Cell Freezing Medium (Sigma C2639) + 10% DMSO or use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Growth Mode

Aggregates in suspension

Additional Info

Depositor

European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury, SP4 0JG, UK

Country of Origin

Spain

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None Specified By Depositor

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MDCK-Protein Free (ECACC 02050101).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.