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MLA144

MLA144

Catalogue No.

86102901

Cell Line Name

MLA144

Cell Line Description

A continuous lymphoid T cell line derived from a Gibbon lymphosarcoma. Cells lack the Fc Ig receptor. Media conditioned by MLA144 supports the growth of T cells from other primates and humans. MLA 144 are positive for the Gibbon ape leukemia virus (GaLV), a simian retrovirus with a moderate oncogenic risk. Handle at ACDP Category 2 containment.

General Info

Species

Monkey

Release Conditions

Restricted - CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the United Kingdom

Characteristics

Products

Interleukin 2 (IL-2)

Tissue of Origin

Lymphoma

Karyotype

Not specified

Disease

Lymphosarcoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr S Clark, PHLS CAMR, Porton Down, Salisbury

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Adv Virus Res 1979;24:1; J Immunol 1981;127:1852; J Cell Physiol 1983;115:191

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MLA144 (ECACC 86102901).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.