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UK Health Security Agency

MOCHA

MOCHA

Catalogue No.

02111901

Cell Line Name

MOCHA

Cell Line Description

Mocha is a naturally occuring mouse mutant with a null mutation in the delta subunit of AP-3 complex. In Mocha cells, the ß3 amd µ3 subunits coassmeble into a heterodimer whereas the sigma 3 subunit remains monomeric. The mouse has a similar phenotype to the human genetic disorder, Hermansky Pudlak Syndrome (HPS). In both mice and humans, AP-3 deficiency results in hypopigmentation because of abnormalities in melanosomes. The Mocha cell line can be used to investigate the assembly of AP-3 complexes and AP-3 function.

Characteristics

Tissue of Origin

Skin

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr M S Robinson University of Cambridge, CIMR, Wellcome Trust, MRC Building, Cambridge. CB2 2XY.

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J. Cell Biol. 2002, 156 p327-336

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MOCHA (ECACC 02111901).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.