MT-2
MT-2
Cell Line Name
Cell Line Description
Derived from a 45 year old Japanese female with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of her peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions. MT-2 has a male karyotype and the presence of a Y chromosome has been confirmed by STR profiling at ECACC
Discontinued Message
Pending internal review.
General Info
Species
Human
Links
Cellosaurus: CVCL_2631
Release Conditions
Characteristics
Products
Tissue of Origin
Karyotype
Applications
MT-4 cells are susceptible to virus infection with HTLV-III and produces viral specific antigens and viral progeny in 4 days.
Disease
Culture Conditions
Cell Type
Subculture Routine
Seed cultures between 2-3x100,000 cells/ml. Saturation density 9 x 100,000 cells/ml; 5% CO₂; 37°C.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Decomplemented Foetal Bovine Serum (FBS).
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Harada et al., (1985) Infection of human T-lymphotropic virus type-1 (HTLV-1)-bearing MT-4 cells with HTLV-III (AIDS virus): chronological studies of early events. Virology, 146: 272-281.
Bibliography
Miyoshi et al., (1982) Gann Monographs 28: 770-771.Harada et al., (1985) Infection of HTLV-III/LAV in HTLV-1-carrying Cells MT-2 and MT-4 and Application in a Plaque Assay. Science 229: 563-566.
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: MT-2 (ECACC 08081401).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.