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UK Health Security Agency

My-La CD4+

My-La CD4+

Catalogue No.

95051032

Cell Line Name

My-La CD4+

Cell Line Description

The continuous cell line My-La CD4+ was established from the independent proliferation of a non-malignant skin homing T cell line from an 82-year old male patient (from a plaque biopsy) with mycosis fungoides stage II by inclusion of IL- 2 and IL-4 in the culture medium.

 

A related cell line was also established from the same patient (My-La Lymphoma, ECACC catalogue No. 95051033).


My-La, CD4+, is also referred to in the literature as My-La, marker chromosome and My-La, Lymphoma is referred to as My-La 46,XY-18+i(1 Bq) or My-La COB+ (ECACC catalogue no. 95051033).


My-La CD4+ is strictly dependent on exogenous IL-2 and IL-4 in the culture medium whereas My-La, Lymphoma on IL-2 only. Both show characteristic chromosome markers and are double homozygous for HLA-A 1.88. My-La, Lymphoma expresses beta-13 of the T cell receptor and phenotypically weak staining was observed for CO2, CD3, COB, CD25, CD71 and TCR-1 alpha/ß.


My-La CD4+ expresses beta-18 of the T cell receptor plus staining for CO2, CD3, CD4,CD25, CD71, HLA-DR and TCR-1 alpha/ B. There has been no evidence of HTLV-1 infection in the cell lines and serum from the patient did not demonstrate antibodies against HTLV-1 and HTLV-11. Both cell lines show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.

Characteristics

Tissue of Origin

Lymph

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11,12
D5S818: 9,12
D7S820: 10,11
D13S317: 11,12
D16S539: 8,11
TH01: 6
TPOX: 8,12
vWA: 17

Karyotype

See reference

Applications

Study of antigen-independent proliferation of T- cell lymphoma from mycosis fungoides patient.

Disease

None Stated

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Split only 1:2 as maximum dilution: freeze in 45% growth medium, 45% human AB serum plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures. Population doubling time 20-24 hours.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10 U/ml IL-2 + 10 U/ml IL-4 + 10% human AB serum

Growth Mode

Suspension

Additional Info

Depositor

Dr K Kaltoft, Institute of Human Genetics, Aarhus University

Country of Origin

Denmark

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Kaltoft et al., 1992 Establishment of two continuous T-cell strains from a single plaque of a patient with mycosis fungoides. In Vitro Cell Dev Biol 28A: 161-167

Bibliography

Thestrup-Pedersenh & Kaltoft 1994 Genotraumatic T cells and cutaneous T-cell lymphoma. A causal relationship? 1994 Arch Dermatol Res 287: 97-101. Kaltoft et al., 1994 Cytogenetic findings in cell lines from cutaneous T-cell lymphoma. Dermatologic Clinics 12: 295-304.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: My-La CD4+ (ECACC 95051032).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.