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UK Health Security Agency

My-La, Lymphoma

My-La, Lymphoma

Catalogue No.

95051033

Cell Line Name

My-La, Lymphoma

Cell Line Description

The cell line My-La, Lymphoma also known as My-La, marker chromosome was established from a plaque biopsy of an 82 year old Caucasian patient with mycosis fungoides stage II. Together with My-La, Lymphoma a second cell line has been established (My-La, CD4+ ECACC catalogue no. 95051032) by including IL-2 and 1L-4 in the culture medium.
My-La, Lymphoma shows characteristic chromosome markers, express ß-13 of the T cell receptor and are double homozygous for HLA-A1.B8. Phenotypically a weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 alpha/ß. There has been no evidence of HTLV-I infection in the cell line and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-11. My-La, Lymphoma cells show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.

Characteristics

Tissue of Origin

Lymph

Karyotype

Not specified

Applications

Study of antigen-independent proliferation of T- cell lyphoma from mycosis fungoides patient.

Disease

Non-Hodgkin Lymphoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Split only 1:2 as maximum dilution: freeze in growth medium plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10 u/ml IL-2 + 10% human AB serum

Growth Mode

Suspension

Additional Info

Depositor

Dr K Kaltoft, Institute of Human Genetics, Aarhus University

Country of Origin

Denmark

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

In Vitro Cell Dev Biol 1992: 28A: 161; Arch Dermatol Res 1994; 287: 97

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: My-La, Lymphoma (ECACC 95051033).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.