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UK Health Security Agency

Mycobacterium smegmatis

Mycobacterium smegmatis

Catalogue No.

NCTC 333

Current Name

Mycobacterium smegmatis

Type Strain

Reference Strain

Original Strain Reference

GP 60

Culture Conditions

Growth Conditions on Solid Media

Glucose agar, 37°C, O₂

Biological and Genomic Info

Fermentation

Glucose: +

Lactose: -

Gram Stain Result

Positive

Enzyme Tests

Catalase: +

Gelatinase: -

Phenylalanone aminotransferase: -

Strain Data

(ATCC 360) F. Griffith, Ministry of Health in 1920 / Redeposition by ATCC in 1932 / Guinea-pig, which died 22 days after inoculation with butter sample No. 15 / Mycobacterium butyricum / Report Local Government Board (Suppl. of Med. Officer) 1912-13, 42, 299 / Gordon, R. E. & Smith, M. M. (1953) J. Bact. 66, 41

Colony Appearance

Rough

Additional Info

Accession Date

01/01/1920

Isolated From

Guinea pig, died 22 days after inoculation with butter sample No 15

History

Isolated by F Griffith, Ministry of Health, 1920.Redeposited by ATCC 1932 PRE:FR

Previous Catalogue Name

Deposited as Mycobacterium butyricum

Applications

Bibliography

GORDON R E & SMITH M M 1953 J BACT 66 41; REPORT LOCAL GOVERNMENT BOARD

Extended Bibliography

Gingeras,T.R, Ghandour,G, Wang,E, Berno,A, Small,P.M, Drobniewski,F, Alland,D, Desmond,E, Holodniy,M, Drenkow,J., (1998), Simultaneous genotyping and species identification using hybridization pattern recognition analysis of generic Mycobacterium DNA arrays, Genome Res, 435-48, https://www.ncbi.nlm.nih.gov/pubmed/9582189 Kim,B.J, Lee,S.H, Lyu,M.A, Kim,S.J, Bai,G.H, Chae,G.T, Kim,E.C, Cha,C.Y, Kook,Y.H., (1999), Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB), J Clin Microbiol, 1714-20, https://www.ncbi.nlm.nih.gov/pubmed/10325313 Brown,B.A, Springer,B, Steingrube,V.A, Wilson,R.W, Pfyffer,G.E, Garcia,M.J, Menendez,M.C, Rodriguez-Salgado,B, Jost KC,J.r, Chiu,S.H, Onyi,G.O, Bottger,E.C, Wallace RJ,J.r., (1999), Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy, Int J Syst Bacteriol, 1493-511, https://www.ncbi.nlm.nih.gov/pubmed/10555330 Adekambi,T, Colson,P, Drancourt,M., (2003), rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria, J Clin Microbiol, 5699-708, https://www.ncbi.nlm.nih.gov/pubmed/14662964 Adekambi,T, Drancourt,M., (2004), Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing, Int J Syst Evol Microbiol, 2095-105, https://www.ncbi.nlm.nih.gov/pubmed/15545441 Brown,B.A, Springer,B, Steingrube,V.A, Wilson,R.W, Pfyffer,G.E, Garcia,M.J, Menendez,M.C, Rodriguez-Salgado,B, Jost KC,J.r, Chiu,S.H, Onyi,G.O, Bottger,E.C, Wallace RJ,J.r., (1999), Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy, Int J Syst Bacteriol, 1493-511, https://www.ncbi.nlm.nih.gov/pubmed/20023030

Available Formats

  • Ampoule (Bacteria)

If use of this culture results in a scientific publication, it should be cited in the publication as: Mycobacterium smegmatis (NCTC 333)

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

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