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UK Health Security Agency

N14A

N14A

Catalogue No.

16110902

Cell Line Name

N14A

Cell Line Description

N14A was establish from a primary pseudomyxoma peritonei (PMP) tissue, (a rare tumour of appendiceal origin) and immortalized with an SV40 T-antigen lentiviral vector (cross-checked for differentially expressed genes, from the array analyses, using qPCR.). This cell line is responsive to mitomycin and oxaliplatin. Immortalized cells exhibit the characteristic "cobblestone"epithelial morphology cells and produce a layer of mucin on the flask (do not stain for MUC2 due to the highly glycosylated nature of the mucin) and have a doubling time of approximately 48 h after clonal expansion. The short tandem repeats (STR) 'finger printing' profile of the cell line was matched to genomic DNA isolated from FFPE samples from the same patients and confirmed as identical sources. Additionally, the STR profiles did not match known cell line profiles from the ATCC (data available from authors on request).

General Info

Species

Human

Unique to ECACC

Yes

Also Known As

N14A, N14-A, N14

Release Conditions

Restricted - all organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

n/a

Products

Responsive to mitomycin-C and oxaliplatin

Tissue of Origin

Appendix

Morphology

Epithelial

DNA profile (STR Profile)

Amelogenin: X, XCSF1PO: 12, 12D13S317: 12, 12D16S539: 8, 16D18S51: 16, 18D21S11:28, 30D3S1358: 15, 18D5S818: 11, 13D7S820:10, 11D8S1179: 10, 14FGA: 23, 24Penta D:12,13penta E: 5, 7THO1: 6, 9TPOX: 8, 8vWA: 16, 17

Karyotype

n/a

Applications

Cell line for pre-clinical anti-tumour drug testing and oncogene activity in Pseudomyxoma peritonei (PMP) tumour biology.

Disease

Primary Appendiceal Disease

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10⁴ cells/cm² using PBS containing 3 mM EDTA and 0.6 mM DTT (incubate at 37°C). Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 days.

Culture Medium

DMEM + 10% FBS + 25 mM HEPES + 5 µg/ml Insulin + 10 mM Glutamine

Growth Mode

Adherent

Additional Info

Depositor

Paterson Institute for Cancer Research, University of Manchester

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Genetic Modification

This cell line was immortalized with an SV40 T-antigen lentiviral vector.

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Roberts DL., et al. Global gene expression in pseudomyxoma peritonei, with parallel development of two immortalized cell lines. Oncotarget. 2015;6(13):10786-10800

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: N14A (ECACC 16110902).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.