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UK Health Security Agency

Nb2-11

Nb2-11

Catalogue No.

97041101

Cell Line Name

Nb2-11

Cell Line Description

Nb2-11 is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. The cells do not express surface immunoglobulin, and their lactogen dependency was confirmed before deposit. Protocols for the use of Nb2-11 cells in bioassays are available from ECACC on request.

Access Cell line profile for this product.

Characteristics

Receptors

Modified prolactin receptor (the Nb2 form)

Tissue of Origin

Thymus/lymph node

Karyotype

42XY,-17,t(2,4)(q22,q24),t(10,11)(q12,q12),+der(15)t(15,?)(p12,?),-18,+der(18)t(18,?)(p12,?)

Applications

In vitro bioassay of lactogenic hormones, Studies of the mechanisms of lactogen induced mitogenesis, Studies into the action of vinca alkaloids, and Studies of tumour progression

Disease

Lymphoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

At resuscitation seed cultures at 3-5 x 100,000 cells/ml. Once established cultures should be seeded at 4x1,000 and 12x1,000 cells/ml for culture periods of 96 and 72 hours respectively. Incubate flasks at 37°C; 5 % CO₂. Check daily. Do not exceed 9x100,000 cells/ml. Avoid using dilutions of cells with fresh medium at greater than 1:10 ratios; culture doubling time 12 hours.

Culture Medium

Fischer's medium (Life Technologies product number : 21475025) + 10%Foetal Bovine Serum (FBS) + 10% Horse Serum (HS) (gelding) + 0.075% Na Bicarbonate + 0.05mM 2-Mercaptoethanol (2ME) + 2mM Glutamine.

Growth Mode

Suspension

Additional Info

Depositor

Dr Peter Gout and Dr Henry Friesen, Department of Cancer Endocrinology, British Columbia Cancer Agency, Vancouver

Country of Origin

Canada

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Gout PW, Beer CT, Noble RL 1980 Prolactin-stimulated growth of cell cultures established from malignant Nb rat lymphomas Cancer Res. 40(7):2433-6 PMID: 6992985.

Bibliography

Horsman DE, Masui S, Gout PW 1991 Karyotypic changes associated with loss of prolactin dependency of rat Nb2 node lymphoma cell cultures. Cancer Res.; 51(1):282-7.PMID: 1988090. Horseman ND 1995 Prolactin, proliferation, and protooncogenes Endocrinology. 136(12):5249-51 PMID: 7588267. Gout PW, Horsman DE, Fox K, De Jong G, Ma S, Bruchovsky N. 1994 The rat Nb2 lymphoma: a novel model for tumor progression. Anticancer Res. 14:2485-92. PMID: 7872671

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Nb2-11 (ECACC 97041101).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.