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NCTC clone 1469

NCTC clone 1469

Catalogue No.

88111403

Cell Line Name

NCTC clone 1469

Cell Line Description

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

Characteristics

Tissue of Origin

Liver

Karyotype

Not specified

Applications

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearance similar to bacterial contamination. Suspension cells are viable and can be used for further subculture.

Culture Medium

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Nat Cancer Inst 1952;12:1245; J Nat Cancer Inst 1957;18:701; J Nat Cancer Inst 1959;23:823

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: NCTC clone 1469 (ECACC 88111403).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.