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UK Health Security Agency

NCTC clone 929

NCTC clone 929

Catalogue No.

85103115

Cell Line Name

NCTC clone 929

Cell Line Description

The parent L strain was derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse by WR Earle in 1940. NCTC clone 929 Clone of strain L (also known as L929) was subsequently derived in March 1948. Strain L was one of the first cell strains to be established in continuous culture, and clone 929 was the first cloned strain developed. Clone 929 was established (by the capillary technique for single cell isolation) from the 95th subculture generation of the parent strain. The cells were treated with 20 methylcholanthrene and produced sarcomas when injected into C3H strain mice.

Characteristics

Tissue of Origin

Adipose

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split semi-confluent cultures 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells /cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Mrs M Freshney, The Beatson Institute, Glasgow

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Earle WR, et al. 1943 Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212.

Bibliography

Sanford KK, et al. 1948 The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246.EARLE WR, SANFORD KK, EVANS VJ, WALTZ HK, SHANNON JE Jr.1951 The influence of inoculum size on proliferation in tissue cultures.J Natl Cancer Inst. 12(1):133-53.PMID:14874126. SANFORD KK, HOBBS GL, EARLE WR.1956 The tumor-producing capacity of strain L mouse cells after 10 years in vitro.Cancer Res. 16(2):162-6.PMID:13293658.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: NCTC clone 929 (ECACC 85103115).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.