NIMP-R14
NIMP-R14
Cell Line Name
Cell Line Description
The cell line is a rat hybridoma (immunogenized by purified BALB/c mouse neutrophils) which produces Anti-RTN4IP1 antibodies, subclass IgG2b.
General Info
Fusion Species
Target (Reactivity)
Unique to ECACC
Release Conditions
Characteristics
Myeloma
210RCY3-Ag123
Immunogen
Purified BALB/c mouse neutrophils
Immunological Donor
Antibody Isotype
Morphology
Tissue of Origin
Culture Conditions
Cell Type
Subculture Routine
Maintain cultures between 3-9 x 10⁵ cells/ml for optimal growth in 5% CO₂/95% air at 37°C.
Culture Medium
DMEM + 20% FBS + L-glutamine
Growth Mode
Additional Info
Depositor
Country of Origin
Applications
Assays
Neutrophils
Hazard Group (ACDP)
References
References
Lopez et al. 1984 J Haematology 57 489 - 494 PMID: 6743568BGalfre et al. 1977 Nature 266 550 - 552. PMID: 558524Mentzel et al. 1999 Kidney International 55 1335 - 1347. PMID: 10200998Tacchini-Cottier et al. 2000 J Immunol 165 2628 - 2636. PMID: 10946291de Vries et al. 2003 J Immunol 170 3883 - 3889. PMID: 12646657Chang et al. 1993 J Leukocyte Biol 53 636 - 639 PMID: 8315347Taylor et al. 2014 Nat Immunol 15(2) 143 - 151 PMID: 24362892
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as NIMP-R14 (ECACC 1807101)
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.