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UK Health Security Agency

NTERA-2 clone D1

NTERA-2 clone D1

Catalogue No.

01071221

Cell Line Name

NTERA-2 clone D1

Cell Line Description

The pluripotent human embryonal carcinoma cell line NTERA-2 clone D1, also known as NT2/D1, is a subclone derived from the parent line NTERA-2. NTERA-2 cells were established from a nude mouse xenograft tumour of TERA-2 cells, which were originally isolated from a lung metastasis from a 22 year old patient with primary embryonal carcinoma of the testis. The subline NTERA-2 clone D1 differentiates into neuronal and other cell types in response to retinoic acid (RA) or hexamethylene bisacetamide (HMBA). Differentiated cells become permissive for human cytomegalovirus (HCMV) or human immunodeficiency virus (HIV). Differentiation with RA as inducer results in glycolipid changes, appearance of neurons and induction of Homeobox (HOX) gene clusters. When NTERA-2 clone D1 cells are passaged at high cell density EC-like cells predominate. However, when cultured at low density many large flat cells differing from typical EC cells are formed.

Characteristics

Tissue of Origin

Testis (lung metastasis)

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,12
D5S818: 9,12
D7S820: 10,12
D13S317: 13
D16S539: 11,12,13
TH01: 9.3
TPOX: 8
vWA: 18,19

Karyotype

Aneuploid

Applications

Regulation of human embryonic differentiation, neuronal studies

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split confluent cultures (approximately 30 x 10⁴ cells/ cm²) by gentle scraping to dislodge cells from the flask surface. Cell sheets/clumps can be dispersed by gently pipetting. Cultures should be maintained at high density by seeding new flasks at a density of at least 6 x 10⁴ cells/ cm². Medium should be renewed every 2-3 days and subcultured at approximately 5 days. As the embryonal cells tend to grow in large clumps and must be removed by gentle scraping , viable counts of only 40-50% are normal on resuscitation and when sub-culturing.

Culture Medium

DMEM containing 4.5 g/L glucose + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should be cryopreserved in 95% FCS:5% DMSO

Growth Mode

Adherent

Additional Info

Depositor

Dr. M. Simpkins, Sir William Dunn School of Pathology, Oxford. Originator: Professor P W Andrews, Department Biomedical Science, University of Sheffield.

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Andrews PW, Damjanov I, Simon D, Banting GS, Carlin C, Dracopoli NC, Fogh J. 1984 Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab Invest. 50(2):147-62. PMID: 6694356

Bibliography

Andrews PW. 1984 Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev Biol. 103(2):285-93. PMID: 6144603. Mavilio F, Simeone A, Boncinelli E, Andrews PW. 1988 Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation. 37(1):73-9. PMID: 2898410. Andrews PW, Nudelman E, Hakomori S, Fenderson BA. 1990 Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR). Differentiation.; 43(2):131-8. PMID: 2373286. Hirka G, Prakash K, Kawashima H, Plotkin SA, Andrews PW, Gonczol E. 1991 Differentiation of human embryonal carcinoma cells induces human immunodeficiency virus permissiveness which is stimulated by human cytomegalovirus coinfection. J Virol. 65(5):2732-5. PMID: 1850047

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: NTERA-2 clone D1 (ECACC 01071221).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.