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OAW42-A

OAW42-A

Catalogue No.

20082613

Cell Line Name

OAW42-A

Cell Line Description

Adriamycin-resistant variant of OAW42, a human ovarian tumour derived from an ascites of patient with ovarian cystadenocarcinoma.

Characteristics

Receptors

PGP, MRP, LRP

Tissue of Origin

Ovary

DNA profile (STR Profile)

Amelogenin: X,X
CSF1PO: 11,11
D3S1358: 15,16
D5S818: 11,11
D7S820: 8,8
D8S1179: 13,13
D13S317: 11,11
D16S539: 12,13
D18S51: 16,21
FGA: 22,25
Penta D: 10,10
Penta E: 12,12
TH01: 6,7
TPOX: 8,11
vWA: 15,16

Applications

Cancer research
Drug resistance

Disease

Tumour

Culture Conditions

Cell Type

Developed from an ascites of patient with ovarian cystadenocarcinoma.

Subculture Routine

Upon resuscitation count cells and seed at the higher end of the seeding density to establish culture. Feed with fresh growth medium the following day.

Trypsinise using Trypsin/EDTA.

Seed cells between 1-4e+4 cells/cm2 and change medium 2-3 times weekly.

8% CO2; 37°C (as medium is a mixture of DMEM/F-12 Ham's).

Freeze cells in 5% DMSO and 95% Foetal Bovine Serum (FBS).

Culture Medium

DMEM/Ham's F12 + 10% FBS

Growth Mode

Adherent

Additional Info

Depositor

Joanne Keenan, Justine Meiller, Martin Clynes National Institute for Cellular Biotechnology, Dublin City University Originator: Alice Redmond, 1990 National Institute for Cellular Biotechnology (formerly National Cell and Tissue Culture Centre), Dublin City University

Country of Origin

Ireland

GMO Status

Not Applicable

Hazard Group (ACDP)

Hazard Group (ACDP) 1

Applications

References

Redmond A, Moran E, Clynes M, 1993 Multiple drug resistance in the human ovarian carcinoma cell line OAW42-A. EJC 29(8);1078-81. PMID 8100138

Bibliography

Moran E et al., 1997. Co-expression of MDR-associated markers, including P-170, MRP and LRP and cytoskeletal proteins, in three resistant variants of the human ovarian carcinoma cell line, OAW42. EJC 33(4);652-60. PMID 9274450

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: OAW42-A (ECACC 20082613).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.