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UK Health Security Agency

OE19

OE19

Catalogue No.

96071721

Cell Line Name

OE19

Cell Line Description

The cell line OE19, also known as JROECL19, was established in 1993 from an adenocarcinoma of gastric cardia/oesophageal gastric junction of a 72 year old male patient. The tumour was identified as pathological stage III (UICC) and showed moderate differentiation. The cell line OE19 expresses HLA-A, -B and -C antigens (MHC class I) constitutively. Treatment with interferon-gamma induced the expression of ICAM-1 (CD54). Expression of HLA-DR (MHC class II) on interferon-gamma addition was only measured in a sub-population of OE19. The cells express epithelial cytokeratins and are tumourigenic in nude mice.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

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Characteristics

Receptors

MHC class I

Tissue of Origin

Oesophagus/gastric cardia

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,13
D5S818: 11,14
D7S820: 8
D13S317: 9,11
D16S539: 12,13
TH01: 8,9
TPOX: 8
vWA: 16,17

Karyotype

Aneuploid

Applications

Study of oesophageal cancer

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 2-4 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.These cells grow slowly in islands and when resuscitated from frozen it can take up to 7 days to reach 70% confluence.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Drs J C Rockett/A Morriss, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Rockett JC, Larkin K, Darnton SJ, Morris AG, Matthews HR 1997 Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization. Br J Cancer. 75(2):258-63 PMID: 9010035

Bibliography

Boonstra JJ, van Marion R, Beer DG, Lin L, Chaves P, Ribeiro C, Pereira AD, Roque L, Darnton SJ, Altorki NK, Schrump DS, Klimstra DS, Tang LH, Eshleman JR, Alvarez H, Shimada Y, van Dekken H, Tilanus HW, Dinjens WN. 2010 Verification and unmasking of widely used human esophageal adenocarcinoma cell lines. J Natl Cancer Inst. 102(4):271-4.PMID: 20075370. Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: OE19 (ECACC 96071721).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.