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UK Health Security Agency

OKT4

OKT4

Catalogue No.

87012602

Cell Line Name

OKT4

Cell Line Description

The antibody is directed against the CD4 antigen on human T cells of the T helper subset.

Characteristics

Myeloma

P3X63 Ag8

Immunogen

Human peripheral blood T lymphocytes

Immunological Donor

CAF1 mouse spleen

Antibody Isotype

IgG2b

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

On resuscitation, a centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at approximately 5 x 105 cells/ml.

Once culture has established, cells should be subcultured when it reaches between 5x105 cells/ml and 9x105 cells/ml (usually after 3-4 days) using a suggested seeding density of 3-4 x105 cells/ml

Recommended growth conditions: 5% CO2, 37°C.

Culture Medium

IDMEM + 2mM glutamine + 20% Foetal Bovine Serum (FBS)

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Patent

This material is cited in a US and/or other Patent and may not be used to infringe patent claims. US Patent No 4,381,295.

Hazard Group (ACDP)

2

References

References

Proc Nat Acad Sci, USA 1980;77:4914

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: OKT4 (ECACC 87012602)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.