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UK Health Security Agency

OX-130

OX-130

Catalogue No.

15090205

Cell Line Name

OX-130

Cell Line Description

The hybridoma cell line OX-130 secretes rat monoclonal IgG2b antibodies reactive with human SIRPβ1 membrane protein.

The production of a monoclonal antibody (MAb) OX-130 recognizes both common alleles of the human activating SIRPβ1 receptor but also cross-reacts with one of the common alleles of the inhibitory human SIRPα receptor. Thus, one might get different outcomes when this MAb is used in assays from different individuals and shows the importance of characterizing SIRP MAb in this way.

General Info

Fusion Species

Mouse Spleen X NS1

Target (Reactivity)

Human SIRPβ1

Also known as

OX130, OX-130

Unique to ECACC

Yes

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Antibody Isotype

IgG2b

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

Seed at 5x104 cells/ml. Subculture when cells reach ~5x105 cells/ml (usually after 3-4 days); 5% CO2; 37°C.

When recovering hybridoma cultures from frozen it is not unusual for growth to be slower than expected initially and there may be an observed decrease in viability. Establishment of an actively proliferating culture may take up to 2 weeks.

On resuscitation a centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of prewarmed growth media+. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Place culture flask flat and observe regularly until viable proliferating cells are seen.

Culture Medium

RPMI 1640 + 4mM glutamine + 10% Foetal Bovine Serum (FBS)

Often hybridoma cultures may benefit from being resuspended with media supplemented with 20% FBS in the early critical stage of culture establishment immediately post resuscitation.

Growth Mode

Suspension

Additional Info

Depositor

Deposited by: LifeArc (MRC Technology), 7th Floor Lynton House, 7-12 Tavistock Square, London, WC1H 9LT Originator: Neil Barclay, Sir William Dunn School of Pathology, University of Oxford, OX1 3RE

Country of Origin

United Kingdom

Applications

Recognises human SIRPβ1 but not SIRPα

Patent

None Specified by Depositor

GMO Status

Not Applicable

Hazard Group (ACDP)

2

References

References

Hatherley D, Aknin ML, Barclay AN. Monoclonal Antibody Recognises Human SIRPb1 but Cross-Reacts on SIRPa from One Allele. Monoc Immunodiagn Immunother. 2016 Feb;35(1):57-9.

Release Conditions

Restricted - Commerical Organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commerical Organisations' release conditions form in the supporting documents.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: OX-130 (ECACC 15090205)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.