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UK Health Security Agency

OX-89

OX-89

Catalogue No.

03062501

Cell Line Name

OX-89

Cell Line Description

This antibody recognises the ligand for OX40 (CD134 ligand) on dendritic cells and some activated lymphocytes.

General Info

Fusion Species

Rat x Mouse Hybridoma

Target (Reactivity)

Anti-OX40 ligand (CD134 ligand), mouse

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Myeloma

NS-1

Immunogen

Con A activated mouse spleen

Immunological Donor

PVG rat

Antibody Isotype

rat IgG1

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at approximately 5 x 105 cells/ml.

Once culture has established, subculture when cells reach 5-9 x 105 cells/ml (usually after 3-4 days). Seed between 5 x 104 and 2 x 105cells/ml.

Recommended growth conditions: 5% CO2, 37°C. 

Culture Medium

RPMI 1640 +2mM glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Suspension

Additional Info

Depositor

Prof. A Neil Barclay, Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford

Country of Origin

United Kingdom

Hazard Group (ACDP)

2

References

References

J Immunol 2001, 166:6972

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: OX-89 (ECACC 03062501)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.