OX-90
OX-90
Cell Line Name
Cell Line Description
This antibody recognises the CD200 antigen present on a variety of cells including activated T lymphocytes, neurons, follicular dendritic cells and endothelium.
General Info
Fusion Species
Target (Reactivity)
Links
Cellosaurus: CVCL_U029
Release Conditions
Characteristics
Myeloma
NS-1
Immunogen
Recombinant mOX-2-rCD4 d3-4
Immunological Donor
Antibody Isotype
Morphology
Tissue of Origin
Culture Conditions
Cell Type
Subculture Routine
A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at approximately 5 x 105 cells/ml.
Once culture has established, subculture when cells reach 5-9 x 105 cells/ml (usually after 3-4 days). Seed between 5 x 104 and 2 x 105cells/ml.
Recommended growth conditions: 5% CO2, 37°C.
Culture Medium
RPMI 1640 +2mM glutamine + 10% Foetal Bovine Serum (FBS)
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
References
References
Science 2000 290:1768
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: OX-90 (ECACC 03062502)
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.