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UK Health Security Agency

PEA-13

PEA-13

Catalogue No.

97040311

Cell Line Name

PEA-13

Cell Line Description

The cell line PEA-13 is homozygous for a disruption of the gene for low density lipoprotein receptor-related protein (LRP). PEA-13 was derived from fibroblasts from day 15 C57BL/6x129 F1 hybrid mouse embryos. Pseudomonas exotoxin A (PEA) was used to select murine fibroblasts genetically deficient in LRP. Together with the cell lines PEA-10 (ECACC catalogue no. 97040310) and MEF-1 (ECACC catalogue no. 98061101) an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LRP-deficiency. This is of importance since LRP-deficient mouse embryos die early during gestation.

Characteristics

Tissue of Origin

Embryo

Applications

Study of LRP and LRP deficiency

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:10 i.e. seeding 2-4 x 10,000 cells / cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Cell Sci. 1994; 107:719

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: PEA-13 (ECACC 97040311).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.