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UK Health Security Agency

RC-4B/C1

RC-4B/C1

Catalogue No.

94020810

Cell Line Name

RC-4B/C1

Cell Line Description

RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.

Characteristics

Receptors

Gonadotrophin-releasing hormone receptors

Products

Gonadatrophs

Tissue of Origin

Pituitary gland

Karyotype

Not specified

Applications

Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.

Disease

Neoplasms

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm² using 0.05% trypsin/EDTA; 10% CO₂; 34°C. Saturation density is reached at 1x 100,000 cells/cm². Cells may attach lightly and so handling should be kept to a minimum. Care should be taken when washing with FBS during subculture. In fact, trypsination may not be necessary as a gentle knock of the flask may detach the cells.

Culture Medium

DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.

Growth Mode

Adherent

Additional Info

Depositor

Dr M Jutisz, Univ Pierre and Marie Curie, CNRS 1449, 75252 Paris

Country of Origin

France

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

In Vitro 1990;26:431; Neuroendocrinol 1991;54:267

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: RC-4B/C1 (ECACC 94020810).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.