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RCC BA85#21 (ELTHEM)

RCC BA85#21 (ELTHEM)

Catalogue No.

13051601

Cell Line Name

RCC BA85#21 (ELTHEM)

Cell Line Description

RCC BA85#21 (ELTHEM) is a renal tumour cell line which was established from a primary tumour in a 62 year-old male patient with a clear cell carcinoma. The tumour tissue of the primary lesion was composed principally of clear cells with alveolar/tubular arrangement. The immunogenicity of the RCC BA85#21 (ELTHEM) cell line has been experimentally proven by in vitro stimulation under autologous and allogeneic conditions using dentritic cells loaded with cell lysate, cytotoxic assay and by the Elispot technique. Characterisation of RCC BA85#21 (ELTHEM) by phenotypic and molecular assays such as cytochemistry, flow cytometry, microarray analysis Real-Time PCR microsatellite instability and loss of heterozygosity is described.

Characteristics

Receptors

High levels of HLA-class I (88.8%) lower levels of CD40 (28%) and CD54 (7.5%)

Tissue of Origin

Kidney

Morphology

Polygonal

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,11
D5S818: 12
D7S820: 10
D13S317: 12
D16S539: 10,12
TH01: 6,8
TPOX: 8,10
vWA: 14,18

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (90%) 1:6 with media changes every 2-3 days. Inoculate new cultures at 4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Professor Elena Ranieri, Universita' degli Studi Di Foggia, Italy.

Country of Origin

Italy

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

2005 Title : "Renal carcinoma cell line and use thereof". Industrial Patent No. MI2005 A002018, 21.10.05. Inventors: E Ranieri, L Gesualdo, W Herr, M Battaglia. Applicants: University of Bari, University of Foggia.

Bibliography

Lichtenfels R, Kellner R, Atkins D, Bukur J, Ackermann A, Beck J, Brenner W, Melchior S, Seliger B. 2003. Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses. Biochim Biophys Acta. 21; 1646(1-2):21-31. PMID: 12637008. Banchereau J, Steinman RM. 1998 Dendritic cells and the control of immunity. Nature. 19; 392(6673):245-52. PMID: 9521319

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: RCC BA85#21 (ELTHEM) (ECACC 13051601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.