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UK Health Security Agency

REM 134

REM 134

Catalogue No.

12122002

Cell Line Name

REM 134

Cell Line Description

REM 134 is a canine mammary carcinoma cell line derived from a spontaneous primary tumour.

Characteristics

Tissue of Origin

Mammary gland

Morphology

Mixed population of polygonal cells

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) seed new cultures at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Professor RW Else, University of Edinburgh, Royal (Dick) School of Veterinary Studies, Easter Bush Veterinary Centre, Easter Bush, Midlothian, EH25 9RG.

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Else RW, Norval M, Neill WA.1982 The characteristics of a canine mammary carcinoma cell line, REM 134. Br J Cancer.46(4):675-81. PMID: 7138772

Bibliography

Norval M, Maingay J, Else RW.1984 Studies of three canine mammary carcinoma cell lines--I. In vitro properties.Eur J Cancer Clin Oncol. 20(12):1489-500. PMID: 6389148. Norval M, Maingay J, Else RW 1984 Studies of three canine mammary cell lines--II. In vivo properties. Eur J Cancer Clin Oncol. 20(12):1501-8.PMID: 6542015. Norval M, Else RW, Maingay J.1988 The effect of tamoxifen on tumours induced by cells from a canine mammary carcinoma cell line in athymic nude mice.Res Vet Sci. ;44(1):76-81.PMID: 3375592. Penzo C, Ross M, Muirhead R, Else R, Argyle DJ. 2009 Effect of recombinant feline interferon-omega alone and in combination with chemotherapeutic agents on putative tumour-initiating cells and daughter cells derived from canine and feline mammary tumours.Vet Comp Oncol.;7(4):222-9. PMID: 19891692.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: REM 134 (ECACC 12122002).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.