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RENTts3.1

RENTts3.1

Catalogue No.

92092516

Cell Line Name

RENTts3.1

Cell Line Description

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat, transformed with a ts mutant of SV40 T antigen (tsA58). Cells express vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43.

Characteristics

Receptors

Glucocorticoid

Tissue of Origin

Endometrium

Morphology

Cuboidal

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 34°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

Culture Medium

DMEM + 20µg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Growth Mode

Adherent

Additional Info

Depositor

Prof M Beato, Institut fur Molekularbiologie and Tumorforschung, W-3550 Marburg/Lahn

Country of Origin

Germany

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Eur J Cell Biol 1991;56:49

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: RENTts3.1 (ECACC 92092516).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.