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UK Health Security Agency

RPMI 8866

RPMI 8866

Catalogue No.

95041316

Cell Line Name

RPMI 8866

Cell Line Description

RPMI 8866 has been established from the peripheral blood of a 51-year-old American woman with chronic myelogenous leukaemia in May 1966. The B lymphoid cell line expresses a relatively high amount of calcitonin receptors, membrane bound Ig and soluble IgE-binding factor (Fc epsilon RII/CD23).

Characteristics

Receptors

Immunoglobulin E (IgE) binding factor, Calcitonin

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11
D5S818: 12
D7S820: 11,12
D13S317: 10,14
D16S539: 11
TH01: 9.3
TPOX: 8,11
vWA: 14,16

Karyotype

Not specified

Applications

Receptor studies, binding assays, hormone studies

Disease

Myelogenous Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

To keep the cells in exponential growth maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 200µl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37°C; 5 - 7% CO₂. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Immunol 1967;99:839; J Biol Chem 1974;249:6812

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: RPMI 8866 (ECACC 95041316).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.