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UK Health Security Agency

SCL 4.1/F7

SCL 4.1/F7

Catalogue No.

93031204

Cell Line Name

SCL 4.1/F7

Cell Line Description

This cell line was derived from neonatal Wistar rat Schwann cells being cultured for several months with intermittent exposure to the mitogen, cholera toxin and infrequent passaging to avoid premature transformation. After cloning one of these cultures by limiting dilution, the line SCL4.1/F7 was established growing without mitrogen. The cells display a subratum adhesion which is calcium-dependent in the millimolar range and pronounced contact-inhibition of growth. Confluent or subconfluent cultures readily cease proliferation and change to a differentiated (stellate / bipolar) morphology secreting an autocrine antiproliferative factor. Cells have been eradicated from mycoplasma at ECACC. With increasing passage cells have been shown to undergo transformation. It is recommended to growth-arrest cells for a few days before subculture to avoid rapid transformation.

Characteristics

Tissue of Origin

Nerve sheath

Morphology

Flattened or crescent shaped

Karyotype

42,XY / 43,XY+7

Disease

None Stated

Culture Conditions

Cell Type

Glial

Subculture Routine

Split semi-confluent cultures 1:4 using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C Saturation density is 10,000 cells/cm².

Culture Medium

Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr L Haynes, Dr J K Dyer, M Perrins, Dr J Rushton, School of Biological Sciences, University of Bristol, UK

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Haynes LW, Rushton JA, Perrins MF, Dyer JK, Jones R, Howell R. 1994. Diploid and hyperdiploid rat Schwann cell strains displaying negative autoregulation of growth in vitro and myelin sheath-formation in vivo. J Neurosci Methods. 52(2):119-27. PMID: 7967716.

Bibliography

Fooks AR, Schmitz S, Rushton JA, Howe SJ, Graham PD, Godbole V, Stacey G, Clegg JC, Haynes LW.1997. Differentiation of a Schwann cell line expressing reporter genes in the presence and absence of nerve axons. Biochem Soc Trans. 25(3):543S. PMID: 9388757.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SCL 4.1/F7 (ECACC 93031204).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.