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UK Health Security Agency

Sf9 TiterHigh AC free

Sf9 TiterHigh AC free

Catalogue No.

05011001

Cell Line Name

Sf9 TiterHigh AC free

Cell Line Description

This cell line is a derivative of the Sf9 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf9 (Spodoptera frugiperda) insect cell line. The cell line Sf9 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf9 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.

Characteristics

Tissue of Origin

Ovary

Morphology

Spherical

Karyotype

Not specified

Applications

Virus studies: Baculovirus

Disease

None Stated

Culture Conditions

Subculture Routine

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium. Incubate flask at 27°C; 5% CO₂.Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.Once normal cell growth has been established maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C, 5% CO₂. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.5%DMSO/92.5% 1:1 conditioned media:fresh media.

Culture Medium

TiterHigh Sf Insect Medium (Sigma I5408) (no supplements)

Growth Mode

Semi-adherent

Additional Info

Depositor

Dr. E. Penn, European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury, SP4 0JG, UK

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P. 1977 The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 13(4):213-7 PMID: 68913.

Bibliography

Smith GE, Ju G, Ericson BL, Moschera J, Lahm HW, Chizzonite R, Summers MD. 1985 Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc Natl Acad Sci U S A. 82(24):8404-8 PMID: 3878519.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Sf9 TiterHigh AC free (ECACC 05011001).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.