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SIRC

SIRC

Catalogue No.

89090404

Cell Line Name

SIRC

Cell Line Description

Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.

Characteristics

Tissue of Origin

Cornea

Karyotype

2n = 44

Applications

Virus studies: Rubella virus

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin 0.02% EDTA; 5% CO₂, 37°C.

Culture Medium

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Virus susceptibility: Science 1965;149:633; Proc Soc Exp Med 1966;122:783; Proc Soc Exp Med 1967;125:12

Bibliography

Niederkorn JY1, Meyer DR, Ubelaker JE, Martin JH.,Ultrastructural and immunohistological characterization of the SIRC corneal cell line. In Vitro Cell Dev Biol. 1990 Sep;26(9):923-30. PMID:2228906

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SIRC (ECACC 89090404).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.