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UK Health Security Agency

SIRMu-1

SIRMu-1

Catalogue No.

19091601

Cell Line Name

SIRMu-1

Cell Line Description

Spontaneously Immortalized Rat Müller cell line. Müller cells are a major type of glial cells in the retina of the eye. Strain: Sprague-Dawley This cell line is distributed by ECACC on behalf of CellBank Australia, Children's Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by CellBank Australia. CellBank Australia reference: CBA-1665. NOTE: This cell line may take up to 48hours to seed.

Characteristics

Tissue of Origin

Retina

Morphology

Epithelial-like

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Subcultivation Ratio: 1:5 - 1:10, split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. At thaw, the cells should be seeded at a density of 2-3 x 10⁴cells/cm². After recovery from thaw (48-72 hours), they can be seeded at 0.5-1.0 x 10⁴cells/cm². Flasks will require passaging twice weekly at a 1:10 seeding density. Media renewal: 2-3 times per week. Culture Conditions: Incubate the culture at 37°C with 5% CO₂. Cryoprotectant medium: 10% DMSO + 90% FCS.

Culture Medium

EMEM + 20% Foetal Calf Serum (FCS) + 25mM Glucose + 2mM L-Glutamine

Growth Mode

Adherent

Additional Info

Depositor

Dan Peet - University of Adelaide, South Australia

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Kittipassorn T, Haydinger CD, Wood JPM, Mammone T, Casson RJ, Peet DJ. Characterisation of the novel spontaneously immortalised rat Müller cell line SIRMu-1. Exp Eye Res. 2019 Apr; 181:127-135 PubMed: 30677389

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SIRMu-1 (ECACC 19091601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.