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UK Health Security Agency

SK-N-BE(2)

SK-N-BE(2)

Catalogue No.

95011815

Cell Line Name

SK-N-BE(2)

Cell Line Description

Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.

Characteristics

Tissue of Origin

Neural

Karyotype

Modal no. 44

Applications

Chemotherapy development, chromosome studies, drug resistance, differentiation studies

Disease

Neuroblastoma

Culture Conditions

Cell Type

Epithelial-like/Neuronal-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham's F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr R A Ross and Dr BA Spengler, Department of Biological Sciences, Fordham University, NY

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cancer Res 1978;38:3751; Life Sciences 1991;50:7; Science 1976;191:185; J Natl Cancer Inst 1976;57:683

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SK-N-BE(2) (ECACC 95011815).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.